常用荧光染料的激发及发射波长

荧光素名称激发波长(nm)发射波长(nm)备注
1,5IAEDANS336490 
1,8-ANS372480 
4-Methylumbelliferone385502 
5-carboxy-2,7-dichlorofluorescein504529 
5-Carboxyfluorescein(5-FAM)492518 
5-Carboxynapthofluorescein(pH10)512/598563/668RatioDye,pH
5-Carboxytetramethylrhodamine(5-TAMRA)542568 
5-FAM(5-Carboxyfluorescein)492518 
5-HAT(HydroxyTryptamine)370-415520-540 
5-HydroxyTryptamine(HAT)370-415520-540 
5-ROX(carboxy-X-rhodamine)578 567604 591 
5-TAMRA(5-Carboxytetramethylrhodamine)548 542552 568 
6-Carboxyrhodamine6G518543 
6-CR6G518543 
6-JOE520548 
7-Amino-4-methylcoumarin351430 
7-AminoactinomycinD(7-AAD)546647 
7-Hydroxy-4-methylcoumarin360449,455 
9-Amino-6-chloro-2-methoxyacridine412,430471,474 
ABQ344445 
AcidFuchsin540630 
ACMA(9-Amino-6-chloro-2-methoxyacridine)412,430471,474 
AcridineOrange+DNA502526 
AcridineOrange+RNA460650 
AcridineOrange,bothDNA&RNA440-480520-650 
AcridineRed455-600560-680 
AcridineYellow470550 
Acriflavin436520 
AcriflavinFeulgenSITSA355-425460 
Aequorin(Photoprotein) 466Photoprotein
AFPs-AutoFluorescentProtein-(QuantumBiotechnologies)seesgGFP,sgBFP   
AlexaFluor350346 342442 441 
AlexaFluor430431540 
AlexaFluor488495,492519,520 
AlexaFluor532531,532553,554 
AlexaFluor546556,557572,573 
AlexaFluor568577,578603 
AlexaFluor594590,594617,618 
AlexaFluor633632650 
AlexaFluor647647666 
AlexaFluor660668698 
AlexaFluor680679702 
AlizarinComplexon530-560,580580,624-645 
AlizarinRed530-560580 
Allophycocyanin(APC)630,645655,660 
AMC,AMCA-S345445 
AMCA(Aminomethylcoumarin)345 347425 444 
AMCA-X353442 
AminoactinomycinD555655 
Aminocoumarin346 350442 445 
Aminomethylcoumarin(AMCA)345 347425 444 
AnilinBlue 600 
Anthrocylstearate360-381446 
APC(Allophycocyanin)630,645655,660 
APC-Cy7625-650755 
APTRA-BTC=RatioDye,Zn2+466/380520/530RatioDye,Zn2+
APTS424505 
AstrazonBrilliantRed4G500585 
AstrazonOrangeR470540 
AstrazonRed6B520595 
AstrazonYellow7GLL450480 
Atabrine436490 
ATTO-TAGCBQCA465560 
ATTO-TAGFQ486591 
Auramine460550 
AurophosphineG450580 
Aurophosphine450-490515 
BAO9(Bisaminophenyloxadiazole)365395 
BCECF(highpH)492,503520,528 
BCECF(lowpH)482520 
BerberineSulphate430550 
BetaLactamase409447,520 
BFPblueshiftedGFP(Y66H) BlueFluorescentProtein381,382,383445,447,448blueshiftedGFP(Y66H) BlueFluorescentProtein
BFP/GFPFRET   
Bimane398490 
Bisbenzamide360461 
Bisbenzimide(Hoechst)360461 
bis-BTC=RatioDye,Zn2+455/405529/505RatioDye,Zn2+
BlancophorFFG390470 
BlancophorSV370435 
BOBO-1462481 
BOBO-3570602 
Bodipy492/515490515 
Bodipy493/503533549 
Bodipy500/510509515 
Bodipy505/515502510 
Bodipy530/550528547 
Bodipy542/563543563 
Bodipy558/568558569 
Bodipy564/570564570 
Bodipy576/589579590 
Bodipy581/591584592 
Bodipy630/650-X625642 
Bodipy650/665-X647665 
Bodipy665/676605676 
BodipyFl504,505511,513 
Bodipy FLATP505514 
BodipyFl-Ceramide505511 
BodipyR6GSE528547 
BodipyTMR542574 
BodipyTMR-Xconjugate544573 
BodipyTMR-X,SE544570 
BodipyTR589617 
BodipyTRATP591620 
BodipyTR-XSE588616 
BO-PRO-1462481 
BO-PRO-3544570 
BrilliantSulphoflavinFF430520 
BTC-RatioDyeCa2+464/401533/529RatioDyeCa2+
BTC-5N-atioDye,Zn2+459/417517/532RatioDye,Zn2+
Calcein494517 
CalceinBlue373440 
CalciumCrimson588,589611,615 
CalciumGreen501,506531 
CalciumGreen-1Ca2+Dye506531Ca2+Dye
CalciumGreen-2Ca2+506/503536Ca2+
CalciumGreen-5NCa2+506532Ca2+
CalciumGreen-C18Ca2+509530Ca2+
CalciumOrange549575 576 
CalcofluorWhite385,395,405437,440,445 
Carboxy-X-rhodamine(5-ROX)576601 
CascadeBlue377 398 399420 423 
CascadeYellow399 400550 552 
Catecholamine410470 
CCF2(GeneBlazer)   
CFDA494520 
CFP-CyanFluorescentProtein430,433,436,(453)474,475,476,(501)CyanFluorescentProtein
CFP/YFPFRET   
Chlorophyll480650 
ChromomycinA436-460470 
ChromomycinA445575 
CL-NERF(RatioDye,pH)504/514540RatioDye,pH
CMFDA494520 
CoelenterazineCa2+Dye,bioluminescence(429)465Ca2+Dye,bioluminescence, nativemolecule
Coelenterazinecp(Ca2+Dye,)(430)442Ca2+Dye,bioluminescence
Coelenterazinef(437)473Ca2+Dye,bioluminescence
Coelenterazinefcp 452Ca2+Dye,bioluminescence
Coelenterazineh(437)464Ca2+Dye,bioluminescence
Coelenterazinehcp(433)444Ca2+Dye,bioluminescence
Coelenterazineip 441Ca2+Dye,bioluminescence
Coelenterazinen(431)467Ca2+Dye,bioluminescence
CoelenterazineO460575 
CoumarinPhalloidin387470 
C-phycocyanine   
CPMMethylcoumarin384469Methylcoumarin
CTC400-450602 
CTCFormazan   
Cy2489506 
 554568 
 581598 
Cy3514 552 554566 570 
 649666 
 675695 
Cy5649666 670 
Cy7710,743767,805 
CyanGFP433(453)475(501) 
cyclicAMPFluorosensor(FiCRhR)500517 
CyQuantCellProliferationAssay480520CellProliferationAssay
Dabcyl453  
Dansyl340578 
DansylAmine337517 
DansylCadaverine335518 
DansylChloride372518 
DansylDHPE336517 
Dansylfluoride356none 
DAPI359461 
Dapoxyl403580 
Dapoxyl2374574 
Dapoxyl3373574 
DCFDA504529 
DCFH(DichlorodihydrofluoresceinDiacetate)505535 
DDAO463607 
DHR(Dihydorhodamine123)505534 
Di-4-ANEPPS496705 
Di-8-ANEPPS(non-ratio)488 498605 713 
DiA(4-Di-16-ASP)456591 
DichlorodihydrofluoresceinDiacetate(DCFH)505535 
DiD-LipophilicTracer644665LipophilicTracer
DiD(DiIC18(5))644665 
DIDS341415 
Dihydorhodamine123(DHR)505535 
DiI(DiIC18(3))549,551565 
Dinitrophenol349  
DiO(DiOC18(3))484,487501,502 
DiR748780LipophilicTracer
DiR(DiIC18(7))750779 
DM-NERF(highpH)497/510540RatioDye,pH
DNP349  
Dopamine340490-520 
DsRed558583Redfluorescentprotein
DTAF494520 
DY-630-NHS621660Hemicyane labelforproteinsandDNA
DY-635-NHS634664Hemicyane labelforproteinsandDNA
EBFP383447EnhancedBlueFluorescentProtein
ECFP436474EnhancedCyanFluorescentProtein
EGFP488,498507,516EnhancedGreenFluorescentProtein
ELF97345530 
Eosin524545 
Erythrosin529,532554,555 
ErythrosinITC529555 
EthidiumBromide510,523595,605 
Ethidiumhomodimer-1(EthD-1)528617 
Euchrysin430540 
EukoLight   
Europium(III)chloride   
EYFP513,520527,532EnhancedYellowFluorescentProtein
FastBlue360440 
FDA494520 
Feulgen(Pararosaniline)570625 
FIF(FormaldehydInducedFluorescence)405433 
FITC490,494520,525 
FITCAntibody493517 
FlazoOrange375-530612 
Fluo-3480–506,506520,527 
Fluo-4494516 
Fluorescein(FITC)490,494520,525 
FluoresceinDiacetate494520 
Fluoro-Emerald495524 
Fluoro-Gold(Hydroxystilbamidine)361536 
Fluor-Ruby555582 
FluorX494520 
FM1-43479598 
FM4-46515640 
FuraRed(highpH)572657 
FuraRed/Fluo-3   
Fura-2,highcalcium335505Excitationratiodye
Fura-2,lowcalcium363512Excitationratiodye
Fura-2/BCECF   
GenacrylBrilliantRedB520590 
GenacrylBrilliantYellow10GF430485 
GenacrylPink3G470583 
GenacrylYellow5GF430475 
GeneBlazer(CCF2)   
GFP(S65T)498516 
GFPredshifted(rsGFP)498516 
GFPwildtype,non-UVexcitation(wtGFP)475509 
GFPwildtype,UVexcitation(wtGFP)395509 
GFPuv385508 
GloxalicAcid405460 
GranularBlue355425 
Haematoporphyrin530-560580 
Hoechst33258345487 
Hoechst33342347483 
Hoechst34580392440 
HPTS355465 
Hydroxycoumarin325-360386-455 
Hydroxystilbamidine(FluoroGold)361536 
Hydroxytryptamine400530 
Indo-1,highcalcium330401Emissionratiodye
Indo-1,lowcalcium346475Emissionratiodye
Indodicarbocyanine(DiD)644665 
Indotricarbocyanine(DiR)748780 
IntrawhiteCf360430 
JC-1514529 
JO-JO-1530545 
JO-PRO-1532544 
LaserPro795812 
Laurodan355460 
LDS751(DNA)543712 
LDS751(RNA)590607 
LeucophorPAF370430 
LeucophorSF380465 
LeucophorWS395465 
LissamineRhodamine572,577591,592 
LissamineRhodamineB577592 
LIVE/DEADKitAnimalCells Calcein/Ethidiumhomodimer494 528517 617formoredetailsrefer to
LOLO-1566580 
LO-PRO-1568581 
LuciferYellow425,428528,536,540 
LysoTrackerBlue373422 
LysoTrackerBlue-White466536 
LysoTrackerGreen504,534511,551 
LysoTrackerRed490516 
LysoTrackerYellow551576 
LysoSensorBlue374424 
LysoSensorGreen442505 
LysoSensorYellow/Blue384540 
MagGreen507531 
MagdalaRed(PhloxinB)524600 
Mag-FuraRed483/427659/631RatioDye,Mg2+
Mag-Fura-2369/329 369/330508 511/491RatioDyeCa2+ RatioDyeMg2+
Mag-Fura-5369/330 369/332505/500 505/482RatioDye,Ca2+ RatioDye,Mg2+
Mag-Indo-1349/328 349/330480/390 480/417RatioDye,Ca2+ RatioDye,Mg2+
MagnesiumGreen506,507531 
MagnesiumOrange550575 
MalachiteGreen628  
MarinaBlue362459 
MaxilonBrilliantFlavin10GFF450495 
MaxilonBrilliantFlavin8GFF460495 
Merocyanin555578 
Methoxycoumarin360410 
MitotrackerGreenFM490516 
MitotrackerOrange551576 
MitotrackerRed578599 
Mitramycin450470 
Monobromobimane398490 
Monobromobimane(mBBr-GSH)398500 
Monochlorobimane380461 
MPS(MethylGreenPyronineStilbene)364395 
NBD466539 
NBDAmine450530 
NileRed515-555,559590,640 
Nitrobenzoxadidole465510-650 
Noradrenaline340490-520 
NuclearFastRed289-530580 
NuclearYellow365495 
NylosanBrilliantIavinE8G460510 
OregonGreen503522 
OregonGreen488-X494517 
OregonGreen503522 
OregonGreen488490,493514,520 
OregonGreen500497517 
OregonGreen514506526 
PacificBlue405455 
Pararosaniline(Feulgen)570625 
PBFI340/380420Excitationratiodye
PE-Cy5488670 
PE-Cy7488755,767 
PerCP488675 
 488710 
PE-TexasRed[Red613]488613 
PhloxinB(MagdalaRed)524600 
Phorwite AR360430 
PhorwiteBKL370430 
PhorwiteRev380430 
PhorwiteRPA375430 
Phosphine3R465565 
PhotoResist365610 
PhycoerythrinB[PE]546-565575 
PhycoerythrinR[PE]565578 
PKH26(Sigma)551567 
PKH67496520Chroma
PMIA341376 
PontochromeBlueBlack535-553605 
POPO-1433457 
POPO-3533574 
PO-PRO-1435455 
PO-PRO-3539567 
Primuline410550 
ProcionYellow470600 
PropidiumIodid(PI)(305),536,538617 
PyMPO412,415561,564,570 
Pyrene360387 
Pyronine410540 
PyronineB540-590560-650 
PyrozalBrilliantFlavin7GF365495 
QSY7560  
QuinacrineMustard440510 
Red613[PE-TexasRed]488613 
Resorufin571584,585 
RH414532716 
Rhod-2552576 
Rhodamine550573 
Rhodamine110496,497520 
Rhodamine123507529 
Rhodamine5GLD470565 
Rhodamine6G525555 
RhodamineB540625 
RhodamineB200523-557595 
RhodamineBextra550605 
RhodamineBB540580 
RhodamineBG540572 
RhodamineGreen502527 
RhodaminePhallicidine558,542575,565 
RhodaminePhalloidine542565 
RhodamineRed570590 
RhodamineWT530555 
RoseBengal525,540550-600 
R-phycocyanine   
R-phycoerythrin(PE)565578 
rsGFP473509redshiftedGFP(S65T)
S65A471504 
S65C479507 
S65L484510 
S65T488511 
SapphireGFP395511 
SBFI340/380420Excitationratiodye
Serotonin365520-540 
SevronBrilliantRed2B520595 
SevronBrilliantRed4G500583 
SevronBrilliantRedB530590 
SevronOrange440530 
SevronYellowL430490 
sgBFP387450 
sgBFP(superglowBFP)387450Quantum’sSuperGloGFP AFPs
sgGFP474488 
sgGFP(superglowGFP)474509Quantum’sSuperGloGFP AFPs
SITS336436IonChannels
SITS(Primuline)395-425450 
SITS(StilbeneIsothiosulphonicAcid)365460 
SNAFLcalcein506/535535/620RatioDye,pH
SNAFL-1508/540543/623RatioDye,pH
SNAFL-2514/543546/630RatioDye,pH
SNARFcalcein552/574590/629RatioDye,pH
SNARF1576/548635/587Excitationandemissionratiodye
SodiumGreen506,507532Na+,K+
SpectrumAqua433,/53480/55Vysis
SpectrumGreen497/30,509/31538/44,524/56Vysis
SpectrumOrange559/38,560588/48Vysis
SpectrumRed587,587/35612,612/51 
SPQ(6-methoxy-N-(3-sulfopropyl)quinolinium)344443 
Stilbene335440 
SulphorhodamineBcanC520595 
SulphorhodamineGExtra470570 
SYTO11508,510527,530DyeforDNA,RNA
SYTO12499,500522,519DyeforDNA,RNA
SYTO13488,491509,514DyeforDNA,RNA
SYTO14517,521549,547DyeforDNA,RNA
SYTO15516,518546,555DyeforDNA,RNA
SYTO16488,494518,525DyeforDNA,RNA
SYTO17621634DyeforDNA
SYTO18490,493507,527DyeforDNA,RNA
SYTO20512530DyeforDNA
SYTO21494517DyeforDNA
SYTO22515535DyeforDNA
SYTO23499520DyeforDNA
SYTO24490515DyeforDNA
SYTO25521556DyeforDNA
SYTO40420441DyeforDNA
SYTO41430454DyeforDNA
SYTO42433460DyeforDNA
SYTO43436467DyeforDNA
SYTO44446471DyeforDNA
SYTO45452484DyeforDNA
SYTO59622645DyeforDNA
SYTO60652678DyeforDNA
SYTO61628645DyeforDNA
SYTO62652676DyeforDNA
SYTO63657673DyeforDNA
SYTO64599619DyeforDNA
SYTO80531545NucleicAcidStain
SYTO81530544NucleicAcidStain
SYTO82541560NucleicAcidStain
SYTO83543559NucleicAcidStain
SYTO84567582NucleicAcidStain
SYTO85567583NucleicAcidStain
SYTOXBlue445470NucleicAcidStain
SYTOXGreen504523NucleicAcidStain
SYTOXOrange547570NucleicAcidStain
Tetracycline390-425525-560 
Tetramethylrhodamine(TRITC)555576 
TexasRed595620 
TexasRed-Xconjugate595615 
Thiadicarbocyanine(DiSC3)651,653674,675 
ThiazineRedR596615 
ThiazoleOrange510530 
Thioflavin5430550 
ThioflavinS430550 
ThioflavinTCN350460 
Thiolyte370-385477-488 
ThiozoleOrange453480 
TinopolCBS(CalcofluorWhite)390430 
TMR550573 
TO-PRO-1515531 
TO-PRO-3644657 
TO-PRO-5747770 
TOTO-1514531,533 
TOTO-3642660 
TriColor(PE-Cy5)(488)650667 
TRITCTetramethylRodamineIsoThioCyanate550573 
TrueBlue365425 
TruRed490695 
Ultralite656678 
UranineB420520 
UvitexSFC365435 
wtGFP395(475)508wildtypeGFP
WW781605639 
X-Rhodamine580605 
XRITC582601 
XyleneOrange546580 
Y66F360508 
Y66H360442 
Y66W436485 
YellowGFP513527Yellowshifted GreenFluorescentProtein
YFP513,520527,532YellowFluorescentProtein
YO-PRO-1491506 
YO-PRO-3613629 
YOYO-1491508,509 
YOYO-3612631 
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